Drug Testing Services in Chicago, IL.

Chicago-based since 2014. DOT and non-DOT programs, on-site collections, and 20,000+ collection sites when you need them nationwide.

Walk-ins welcome.

4.9 | 700+ Google Reviews

For Employers

Employer Drug Testing Programs in Chicago

Alia is a certified C/TPA based in Chicago. We manage pre-employment screening, random pools, DOT compliance, post-accident testing, and on-site collections for employers across Illinois and nationwide — including Chicago’s transportation, logistics, construction, manufacturing, and warehouse operations. If you hire, we can support your program.

We work with Quest, LabCorp, Alere, and CRL through eScreen, FormFox, and I3 — the same labs and portals as the large national providers, with the flexibility to match the right platform to your program. The difference is you have a direct contact who knows your account and picks up when something needs attention.

QuestLabCorpAlereCRL eScreenFormFoxI3
For Individuals

Individual Drug Testing in Chicago

Order online, enter your ZIP code, and we’ll place you at a certified collection site near you. With access to certified partner laboratories across the Chicago area and suburbs, we make scheduling fast and straightforward.

You can also walk in or schedule at our Chicago location at 7036 W Belmont Ave.

  • Court orders & probation
  • Employment screening
  • Child custody testing
  • Personal testing — same-day options available

Walk-ins welcome.

Visit Us

Headquartered in Chicago at 7036 W Belmont Ave — serving employers and individuals across the city, suburbs, and beyond.

Walk-ins are welcome during business hours at our Chicago office. Need testing somewhere else? Enter your ZIP code when ordering and we’ll connect you with a certified collection site near you. Through our nationwide network of more than 20,000 collection sites, we coordinate testing across Illinois and throughout the United States while providing local support from our Chicago team.

Whether you’re in downtown Chicago, the North Shore, the western suburbs, Northwest Indiana, or anywhere else in the United States, we make drug and alcohol testing easy to coordinate.

Address

7036 W Belmont Ave, Chicago, IL 60634

Phone

708-320-2334

Hours

Mon–Thur: 9:00 AM – 4:00 PM | Fri 9:00 AM – 3:00 PM

Ready to Get Started?

Drug Testing in Chicago — Common Questions

For Employers
Can Illinois employers still test for marijuana after legalization?

Yes. Illinois legalized recreational cannabis in 2020 but employers retain the right to enforce a zero-tolerance drug-free workplace policy. You can decline to hire or take disciplinary action based on a positive THC result as long as your written policy clearly states it. One important nuance — Illinois law requires that employment decisions be based on actual policy violation, not just impairment alone. DOT-regulated employees are a different story entirely — federal zero-tolerance rules apply regardless of state law, with no exceptions.

Yes, and most employers don’t know this. Illinois allows employers to use a positive drug test result as an intoxication defense against a workers’ compensation claim. A positive test can also make an employee ineligible for unemployment compensation. To leverage either benefit, your program must be set up and documented correctly before an incident occurs — not after. This is one of the strongest financial reasons Chicago employers implement a formal drug testing program.

DOT testing applies to employees in federally regulated safety-sensitive positions — CDL drivers, transit workers, pipeline operators, and others under FMCSA, FTA, FAA, or PHMSA rules. The test type, collection process, lab certification, and MRO review are all federally mandated under 49 CFR Part 40. Non-DOT testing gives employers more flexibility in panel selection and program design, but best practice is to follow the same chain-of-custody standards. If you’re unsure whether your employees are DOT-covered, that’s the first thing to clarify before setting up a program.

Illinois doesn’t mandate drug testing for most private employers, but you should not test without a written policy in place. Without one, a positive result is difficult to act on and creates liability exposure. A written policy defines what you test for, when testing occurs, and what the consequences of a positive result are. It also protects you under Illinois workers’ comp and unemployment law. If you don’t have one, we can help you build it as part of your program setup.

For Individuals
Where can I get a drug test in Chicago without an appointment?

Walk-ins are welcome at our Chicago location at 7036 W Belmont Ave during business hours — Monday through Thursday 9am–4pm, Friday 9am–3pm. If you’re not near our location, order online at aliadrugtesting.com, enter your ZIP code, and we’ll place you at a certified collection site near you across the Chicago area and suburbs. Same-day testing is available at most locations.

For standard lab-based urine tests, negative results typically come back within 24–48 hours. Non-negative results go through MRO review, which can add one to two business days. Instant tests provide a preliminary negative result on the spot — non-negatives require lab confirmation and MRO review before a final result is issued. Hair tests generally take two to three business days. If you need results fast, let us know when you order and we’ll match you with the right test type and location.

For more detailed employer program questions, visit our FAQ for Employers. For individual testing questions, visit our FAQ for Individuals.

Certified Collection Sites in Chicago and the Surrounding Area

We have 30+ locations in Chicago alone, with hundreds more across Chicagoland and 20,000+ nationwide. Order online, enter your ZIP, and we'll match you to the nearest location automatically.

★ Alia Drug Testing — Walk-ins Welcome

7036 W Belmont Ave, Chicago, IL 60634

Mon–Thur: 9:00 AM – 4:00 PM Fri 9:00 AM – 3:00 PM

233 E Erie St, Suite 602

Chicago, IL 60611

Mon–Fri: 9:00 AM – 4:00 PM (Lunch 11–1)

30 N Michigan Ave, Suite 1410

Chicago, IL 60602

Mon–Fri: 9:00 AM – 4:00 PM (Lunch 11–2) | Sat: 9:00 AM – 11:00 AM

825 S State St

Chicago, IL 60605

Mon–Fri: 8:00 AM – 7:00 PM | Sat–Sun: 8:00 AM – 7:00 PM

600 W Adams St

Chicago, IL 60661

Mon–Fri: 8:00 AM – 7:00 PM | Sat–Sun: 8:00 AM – 7:00 PM

614 W Monroe St

Chicago, IL 60661

Mon–Fri: 8:00 AM – 5:00 PM

1230 W Lake St

Chicago, IL 60607

Mon–Fri: 8:00 AM – 5:00 PM

424 W Division St

Chicago, IL 60610

Mon–Fri: 6:00 AM – 2:00 PM | Sat: 7:00 AM – 1:00 PM

121 W North Ave

Chicago, IL 60610

Mon–Fri: 8:00 AM – 7:00 PM | Sat–Sun: 8:00 AM – 7:00 PM

1030 W Chicago Ave

Chicago, IL 60642

Mon–Fri: 7:00 AM – 7:00 PM | Sat: 8:00 AM – 5:00 PM

1460 N Halsted St, Suite 201

Chicago, IL 60642

Mon–Fri: 8:00 AM – 4:30 PM | Sat: 8:00 AM – 1:00 PM

2077 N Clybourne Ave

Chicago, IL 60614

Mon–Fri: 8:00 AM – 7:00 PM | Sat–Sun: 8:00 AM – 7:00 PM

933 W Diversey Pkwy

Chicago, IL 60614

Mon–Fri: 8:00 AM – 7:00 PM | Sat–Sun: 8:00 AM – 7:00 PM

3046 N Halsted St

Chicago, IL 60657

Please call for hours

1702 N Milwaukee Ave

Chicago, IL 60647

Mon–Fri: 8:00 AM – 7:00 PM | Sat–Sun: 8:00 AM – 7:00 PM

3235 W Montrose Ave

Chicago, IL 60618

Mon–Fri: 9:00 AM – 6:00 PM | Sat: 8:00 AM – 12:00 PM

3909 N Western Ave

Chicago, IL 60618

Mon–Fri: 8:00 AM – 7:00 PM | Sat–Sun: 8:00 AM – 7:00 PM

4126 N Milwaukee Ave

Chicago, IL 60641

Mon–Fri: 7:00 AM – 3:00 PM | Sat: 7:00 AM – 12:00 PM

4211 N Cicero Ave

Chicago, IL 60641

Mon–Fri: 8:00 AM – 7:00 PM | Sat–Sun: 8:00 AM – 7:00 PM

4801 W Peterson Ave, Suite 205

Chicago, IL 60646

Mon–Fri: 9:30 AM – 3:00 PM | Sat: 9:30 AM – 12:00 PM

5228 N Northwest Hwy

Chicago, IL 60630

Mon–Fri: 8:00 AM – 7:00 PM | Sat–Sun: 8:00 AM – 7:00 PM

5300 Long Ave

Chicago, IL 60638

Mon–Fri: 8:00 AM – 7:00 PM | Sat–Sun: 8:00 AM – 7:00 PM

5406 W Fullerton Ave

Chicago, IL 60639

Mon–Fri: 7:00 AM – 7:00 PM | Sat: 8:00 AM – 12:00 PM

5961 N Lincoln Ave, Unit 2

Chicago, IL 60659

Mon–Fri: 8:00 AM – 7:00 PM | Sat–Sun: 8:00 AM – 7:00 PM

6140 N Broadway St

Chicago, IL 60660

Mon–Fri: 8:00 AM – 7:00 PM | Sat–Sun: 8:00 AM – 7:00 PM

3145 S Ashland Ave, Suite 110

Chicago, IL 60608

Mon–Fri: 7:00 AM – 6:00 PM

4011 W 63rd St

Chicago, IL 60629

Mon–Fri: 9:00 AM – 4:00 PM (Lunch 11–2)

5015 W 65th St

Chicago, IL 60638

Mon–Fri: 8:00 AM – 5:00 PM

6500 W 65th St

Chicago, IL 60638

Mon–Fri: 8:00 AM – 5:00 PM

7050 S Cicero Ave

Chicago, IL 60638

Mon–Fri: 7:30 AM – 3:00 PM | Sat: 7:30 AM – 1:00 PM

900 E 103rd St

Chicago, IL 60628

Mon–Fri: 8:00 AM – 5:00 PM

10834 S Doty Ave

Chicago, IL 60628

Mon–Fri: 8:00 AM – 7:00 PM | Sat–Sun: 9:00 AM – 5:00 PM

2008 W 119th St, Suite 7

Chicago, IL 60643

Mon–Fri: 8:30 AM – 8:00 PM | Sat: 8:30 AM – 6:00 PM

Need help finding a location near you? Call us at 708-320-2335 or order online and we'll get you set up.